Journal: Cell Death Discovery

Article Title: New AKT-dependent mechanisms of anti-COVID-19 action of high-CBD Cannabis sativa extracts

doi: 10.1038/s41420-022-00876-y

Figure Lengend Snippet: A Western blot analysis of ACE2 and TMPRSS2 in WI-38 cells treated with the indicated most abundant compounds in extract #1, with the same concentration found in the extract; relative densitometry was measured using ImageJ. B Western blot analysis of ACE2 and TMPRSS2 in BJ-5ta cells treated with the indicated most abundant compounds in extract #7, with the same concentration found in the extract; relative densitometry was measured using ImageJ. C Western blot analysis of ACE2 and TMPRSS2 in WI-38 cells treated with either CBD or PTWT2.2 individually or in combination; relative densitometry was measured using ImageJ. D Western blot analysis of ACE2 and TMPRSS2 in WI-38 cells treated with either CBD or PTWT1.2 individually or in combination; relative densitometry was measured using ImageJ. PTWT represents terpene. * indicates p < 0.05; ** indicates p < 0.01.

Article Snippet: Human normal foreskin fibroblasts (BJ-5ta), purchased from American Type Culture Collection (ATCC, Manassas, USA), were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS).

Techniques: Western Blot, Concentration Assay

Journal: Antioxidants

Article Title: Extracts from Cephalaria Uralensis (Murray) Roem. & Schult. and Cephalaria Gigantea (Ledeb.) Bobrov as Potential Agents for Treatment of Acne Vulgaris: Chemical Characterization and In Vitro Biological Evaluation

doi: 10.3390/antiox9090796

Figure Lengend Snippet: Viability of normal human skin fibroblasts (BJ cell line, ATTC CRL-2522 ™ ) after 24 h incubation with the most active extracts (CUA, CUM, CUE, CUMf, CUEf). * Significantly different results compared to control (culture medium without extracts—0 μg/mL); unpaired t -test, p < 0.05.

Article Snippet: Cytotoxicity assessment was carried out using normal human skin fibroblasts (BJ cell line, ATCC CRL-2522 TM ), as described in detail previously [ ].

Techniques: Incubation, Control

Journal: Scientific Reports

Article Title: Evidence for bystander signalling between human trophoblast cells and human embryonic stem cells

doi: 10.1038/srep11694

Figure Lengend Snippet: ( a ) Protocol for indirect exposure of target cells (fibroblasts or hES cells) to Co and Cr ions. ( b ) Protocol for direct exposure of target cells (fibroblasts or hES cells) to Co and Cr ions. ( c ) Analysis of DNA damage in the BeWo bi-layer and fibroblast cells using the alkaline comet assay. Three hundred cells (three repetitions of 100) were analysed at random per parameter per experiment. All experiments were repeated three times giving a total of 900 cells scored per parameter. ( d ) Measurement of total Co and Cr concentration in conditioned medium using inductively coupled plasma mass spectrometry (ICP-MS). Conditioned medium was collected from below nine BeWo barriers and from nine placenta explants (taken from three placentae). The data was compared by one-way ANOVA. When a P value of >0.05 was found post hoc Dunnett’s tests were used to compare each treatment group to the negative control. *P > 0.05, **P > 0.01, ***P > 0.001 when compared to the negative control. Centre values represent means. Error bars represent SEM.

Article Snippet: Primary human BJ skin fibroblasts (LGC Promochem UK) were maintained in T-75 flasks (Corning) in Minimal Essential Medium (Sigma-Aldrich) supplemented with 10% (v/v) foetal bovine serum (Gibco), 1% (v/v) antibiotic antimitotic solution (containing 1000 units penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL, Sigma Aldrich), 0.1 mg/mL sodium pyruvate, 0.02M HEPES buffer and 2 mM L-glutamine (all from Sigma-Aldrich) at 5% CO 2 and 37 °C.

Techniques: Alkaline Single Cell Gel Electrophoresis, Concentration Assay, Clinical Proteomics, Mass Spectrometry, Negative Control